|
KMID : 0352720170410040540
|
|
Journal of Ginseng Research
2017 Volume.41 No. 4 p.540 ~ p.547
|
|
|
Biotransformation of Panax ginseng extract by rat intestinal microflora: identification and quantification of metabolites using liquid chromatography-tandem mass spectrometry
|
|
Dong Wei Wei
Zhao Jin Hua
Zhong Fei Liang
Zhu Wen Jing
Jiang Jun
Wu Song Quan
Yang Deok-Chun
Li Dong Hao
Quan Lin Hu
|
|
|
Abstract
|
|
|
Background: In general, after Panax ginseng is administered orally, intestinal microbes play a crucial role in its degradation and metabolization process. Studies on the metabolism of P. ginseng by microflora are important for obtaining a better understanding of their biological effects.
Methods: In vitro biotransformation of P. ginseng extract by rat intestinal microflora was investigated at 37°C for 24 h, and the simultaneous determination of the metabolites and metabolic profile of P. ginseng saponins by rat intestinal microflora was achieved using LC?MS/MS.
Results: A total of seven ginsenosides were detected in the P. ginseng extract, including ginsenosides Rg1, Re, Rf, Rb1, Rc, Rb2, and Rd. In the transformed P. ginseng samples, considerable amounts of deglycosylated metabolite compound K and Rh1 were detected. In addition, minimal amounts of deglycosylated metabolites (ginsenosides Rg2, F1, F2, Rg3, and protopanaxatriol-type ginsenosides) and untransformed ginsenosides Re, Rg1, and Rd were detected at 24 h. The results indicated that the primary metabolites are compound K and Rh1, and the protopanaxadiol-type ginsenosides were more easily metabolized than protopanaxatriol-type ginsenosides.
Conclusion: This is the first report of the identification and quantification of the metabolism and metabolic profile of P. ginseng extract in rat intestinal microflora using LC?MS/MS. The current study provided new insights for studying the metabolism and active metabolites of P. ginseng.
|
|
|
KEYWORD
|
|
|
ginsenoside, LC?MS/MS, metabolites, Panax ginseng, rat intestinal microflora
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|